Peptide Design for Targeting BTB Domain Homodimerization of BACH2: Complementary In Silico and In Vitro Approaches

using supernatant depletion assay, with peptide 13 showing a trend toward higher affinity. Additionally, the inhibitory effect of peptides on homodimerization of the BACH2 BTB domain was investigated through coimmunoprecipitation studies, revealing peptide 13 as a potential disruptor of homodimerization. Based on these observations, peptide 13 has been identified as a putative BACH2 negative regulator. Furthermore, limitations and future directions are also discussed.