plays a critical role in TPI1 nuclear localization and HIV-1 transcription. Consistently, nuclear TPI1 levels were markedly reduced under galactose conditions, in S80A-expressing cells, or upon TPI1 knockdown. Reduced nuclear TPI1 levels were associated with decreased H3K27 acetylation and diminished HIV-1 transcriptional activity. Moreover, TPI1 localization was unaffected by metabolic conditions in uninfected PBMCs but showed nuclear translocation under aerobic glycolytic conditions in HIV-1-infected PBMCs. Collectively, these findings suggest that, in HIV-1-infected cells, aerobic glycolytic conditions promote TPI1 nuclear translocation, thereby linking cellular metabolism to HIV-1 transcription.
Abe et al. (Fri,) studied this question.