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Investigate the biocontrol potential of Paenibacillus sp. TRMB57781 against pear fire blight and elucidate the underlying molecular mechanisms.

May 24, 2026Open Access

Antagonistic Activity and Molecular Insights into the Biocontrol of Korla Pear Fire Blight by Paenibacillus sp. TRMB57781

Key Points

Investigate the biocontrol potential of Paenibacillus sp. TRMB57781 against pear fire blight and elucidate the underlying molecular mechanisms.
Strain TRMB57781 was isolated from Alar chemical plant wastewater and identified through 16S rRNA gene analysis.
Whole-genome sequencing was conducted to identify a biosynthetic gene cluster for fusaricidin B.
Fermentation medium was optimized, and in vitro efficacy of the broth was tested on fragrant pear inflorescences and detached leaf assays.
A 1:50 dilution of fermentation broth achieved an 84.62% control efficacy against E. amylovora in fragrant pear inflorescences, compared to 69.48% by agricultural streptomycin.
Similar efficacy to streptomycin was observed in detached leaf assays and potted plant trials.
The active compound identified through HR-TOF MS analysis was linked to the antagonistic activity against E. amylovora.

Abstract

Pear fire blight caused by Erwinia amylovora necessitates environmentally friendly biocontrol alternatives. Strain TRMB57781, isolated from Alar chemical plant wastewater, was identified as Paenibacillus sp. via polyphasic taxonomy and 16S rRNA gene analysis. Whole-genome sequencing revealed a biosynthetic gene cluster for fusaricidin B. Fermentation medium optimization with glucose and cottonseed meal significantly enhanced antibacterial activity. In vitro assays demonstrated that a 1:50 dilution of the fermentation broth achieved 84.62% control efficacy on fragrant pear inflorescences, markedly exceeding agricultural streptomycin (69.48%). Comparable efficacy to streptomycin was observed in detached leaf assays and potted plant protective and curative trials. Through HR-TOF MS analysis combined with genomic verification, the active compound was identified as a key antimicrobial metabolite associated with the antagonism against E. amylovora. This study presents Paenibacillus sp. TRMB57781 as a promising biocontrol agent and provides genomic and metabolomic insights into its antibacterial activity against pear fire blight.

Antagonistic Activity and Molecular Insights into the Biocontrol of Korla Pear Fire Blight by Paenibacillus sp. TRMB57781

Key Points

Abstract

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