Clinical Evaluation of a New, Miniaturized Biosensor for Self-monitoring of Blood Glucose

detectable cross-hybridization. The internal amplification controls used in this study were designed to be used as calibrators by which number of input viral template copies could be calculated. A known quantity of the control template was added to the test sample, and a single pair of primers was used to amplify both the viral and control sequences in a single PCR reaction. Under specific amplification conditions, the amount of control PCR product will be proportional to the amount of viral PCR product. The results presented here demonstrate the ability to quantitatively and specifically detect each PCR product in a multiplexed reaction. Preliminary work with clinical HIV samples suggests that multiplexed quantitation is possible.