Synthesis of Phospholipids and Triglycerides in Human Liver Slices I. Experimental Conditions and the Synthesis Rate in Normal Liver Tissue

AbstractHuman liver slices were incubated with glycerol-1,3-14C or fructose-U-14C. 50% of the label was recovered as glucose and 10% was incorporated into CO2 and into lipids, respectively. At high fatty acid concentration in the medium the incorporation into CO2 and glucose was suppressed, into triglycerides increased, and into phospholipids unchanged. The incorporation of fructose was suppressed with glycerol in the medium. The mean synthesis rate for phospholipids was 0.14 ± 0.01 μmoles/g liver per hour and for triglycerides 0.34 ± 0.04 μmoles/g liver per hour.Key Words: Fructose-U-14Clipid synthesisglycerol-1,3-14Clipid synthesisglycerol metabolismliverlipid metabolismliver slicesphospholipid synthesislivertriglyceride synthesisliver