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Abstract Extended countercurrent distribution of yeast phenylalanine transfer RNA (tRNAphe) gives rise to two peaks of phenylalanine acceptor activity. By the use of end group-labeling techniques, the 5'-terminal sequence of each of these peaks has been shown to be pGpC-, and the 3'-terminal sequences are -CpApCpC and -CpApCpCpA for Peaks I and II, respectively. Subsequent to the addition of pA to the 3' terminus of the tRNAphe from Peak I by a crude supernatant enzyme from yeast, this tRNA cannot be distinguished from tRNAphe (Peak II) by countercurrent distribution techniques. It is concluded that the two peaks of phenylalanine acceptor activity observed represent the same species of tRNA and differ only in that the tRNAphe (Peak I) is lacking the 3'-terminal pA residue.
RajBhandary et al. (Thu,) studied this question.
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