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Summary The MP variant of Herpes simplex virus was used in experiments designed to determine the relative rates of spread of virus infection from infected to noninfected FL cells incubated at different temperatures in the presence of antibody. Preliminary experiments showed that one infectious particle is sufficient to infect a cell, and that an infected cell will almost invariably produce a macroplaque when deposited on a monolayer culture and incubated at 37°C in the presence of antibody. The conditions required to effect infection of all or most cells in an FL cell culture were also determined. On the basis of these experiments, the mean size of macroplaques formed by infected cells added to the monolayer culture and incubated in the presence of antibody at 30°, 31°, 34°, 37°, and 39.5°C were determined. From these experiments it was learned that the largest macroplaque area is attained after 4 days of incubation at 37°C. Below and above 37°C the mean size of macroplaques after a similar incubation period is progressively reduced. In view of the absence of the cell fluid-cell transmission of infection in the presence of antibody in the fluid phase, the increase in macroplaques may be taken as a measure of the direct cell-cell transmission of the virus from infected to noninfected cells. The significance of the observed rates of spread of infection at different temperatures with respect to a) virus formation at different temperatures, b) virus release from infected cells at different temperatures, and c) recurrent herpetic infections in man were discussed.
Hoggan et al. (Mon,) studied this question.