Abstract Rosa anemoniflora, a critically endangered climbing rose endemic to China, holds significant ornamental and conservation value but lacks genomic resources. Here, we present a chromosome-level genome assembly generated using PacBio HiFi, Illumina, Iso-Seq, and Hi-C sequencing. The final assembly spans 547. 52 Mb, with 95. 72% of sequences anchored to seven pseudochromosomes and a contig N50 of 73. 49 Mb. BUSCO assessment reveals 97. 36% completeness (eudicotyledonsₒdb12). Repetitive elements constitute 55. 31% of the genome, and 33, 623 protein-coding genes were annotated, 97. 68% of which are supported by BUSCO. We also identified 1, 333 rRNA, 838 tRNA, and 1, 043 other non-coding RNA genes. All data are publicly available at National Genomics Data Center (NGDC) (PRJCA052329). This high-quality genome provides a foundational resource for conservation genomics, evolutionary studies, and the genetic dissection of adaptive and reproductive traits in this threatened species.
Zhou et al. (Mon,) studied this question.