The novel R878C mutation in the cardiac Na+ channel resulted in loss of Na+ currents in vitro, correlating with clinical phenotypes of bradycardia, conduction slowing, and sick sinus syndrome.
Does the novel R878C mutation in the cardiac Na+ channel alter Na+ currents and correlate with clinical phenotypes in a Chinese family?
The novel R878C mutation in the cardiac Na+ channel causes a loss of function that correlates with clinical phenotypes of bradycardia and sick sinus syndrome.
AIM: We compared the clinical and physiological consequences of the novel mutation R878C in a highly conserved pore residue in domain II (S5-S6) of human, hNa(v)1.5, cardiac Na(+) channels. METHODS: Full clinical evaluation of pedigree members through three generations of a Chinese family combined with SCN5A sequencing from genomic DNA was compared with patch and voltage-clamp results from two independent expression systems. RESULTS: The four mutation carriers showed bradycardia, and slowed sino-atrial, atrioventricular and intraventricular conduction. Two also showed sick sinus syndrome; two had ST elevation in leads V1 and V2. Unlike WT-hNa(v)1.5, whole-cell patch-clamped HEK293 cells expressing R878C-hNa(v)1.5 showed no detectable Na(+) currents (i(Na)), even with substitution of a similarly charged lysine residue. Voltage-clamped Xenopus oocytes injected with either 0.04 or 1.5 microg microL(-1) R878C-hNa(v)1.5 cRNA similarly showed no i(Na), yet WT-hNa(v)1.5 cRNA diluted to 0.0004-0.0008 ng microL(-1)resulted in expression of detectable i(Na). i(Na) was simply determined by the amount of injected WT-hNa(v)1.5: doubling the dose of WT-hNa(v)1.5 cRNA doubled i(Na). i(Na) amplitudes and activation and inactivation characteristics were similar irrespective of whether WT-hNa(v)1.5 cRNA was given alone or combined with equal doses of R878C-hNa(v)1.5 cRNA therefore excluding dominant negative phenotypic effects. Na(+) channel function in HEK293 cells transfected with R878C-hNa(v)1.5 was not restored by exposure to mexiletine (200 microM) and lidocaine (100 microM). Fluorescence confocal microscopy using E3-Nav1.5 antibody demonstrated persistent membrane expression of both WT and R878C-hNa(v)1.5. Modelling studies confirmed that such i(Na) reductions reproduced the SSS phenotype. CONCLUSION: Clinical consequences of the novel R878C mutation correlate with results of physiological studies.
Zhang et al. (Tue,) conducted a other in Bradycardia and sick sinus syndrome associated with SCN5A mutation (n=4). R878C mutation in hNav1.5 vs. Wild-type hNav1.5 was evaluated on Clinical phenotype and Na+ current (iNa) characteristics. The novel R878C mutation in the cardiac Na+ channel resulted in loss of Na+ currents in vitro, correlating with clinical phenotypes of bradycardia, conduction slowing, and sick sinus syndrome.