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The U-1 RNA is one of the low molecular weight nuclear RNAs of Novikoff hepatoma ascites cells that is specifically localized to the extranucleolar portion of the nucleus. After the RNA was purified by polyacrylamide gel electrophoresis, it was subjected to various enzymatic digestion procedures for determination of its primary nucleotide sequence. The U-1 RNA contains 171 nucleotides. Its 5′ end is an alkali-stable tetranucleotide containing N2,2,7-trimethyl guanylic acid. The primary sequence of U-1 RNA is: (m32,2,7G, Am, Um) see PDF for sequence
Reddy et al. (Tue,) studied this question.
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