Nanomaterial‐Based Amplified Transduction of Biomolecular Interactions

This article reviews progress in the development of nanomaterials for amplified biosensing and discusses different nanomaterial-based bioamplification strategies. Signal amplification has attracted considerable attention for ultrasensitive detection of disease markers and biothreat agents. The emergence of nanotechnology is opening new horizons for highly sensitive bioaffinity and biocatalytic assays and for novel biosensor protocols that employ electronic, optical, or microgravimetric signal transduction. Nucleic acids and antibodies functionalized with metal or semiconductor nanoparticles have been employed as amplifying tags for the detection of DNA and proteins. The coupling of different nanomaterial-based amplification platforms and amplification processes dramatically enhances the intensity of the analytical signal and leads to ultrasensitive bioassays. The successful realization of the new nanoparticle-based signal amplification strategies requires proper attention to nonspecific adsorption issues. The implications of such nanoscale materials on amplified biodetection protocols and on the development of modern biosensors are discussed.