Unilateral femoral artery ligation in rats significantly increased the rate of glucose utilization in the gastrocnemius and soleus muscles at rest to 126% and 140% of control values, respectively.
In a rat model of arterial insufficiency, skeletal muscle adapts to hypoperfusion by increasing its rate of glucose utilization for a given blood flow.
Absolute Event Rate: 11.5% vs 9.3%
p-value: p=<0.05
Muscle bloodflow and the rate of glucose uptake and phosphorylation were measured in vivo in rats 7 days after unilateral femoral artery ligation and section. Bloodflow was determined by using radiolabelled microspheres. At rest, bloodflow to the gastrocnemius, plantaris and soleus muscles of the ligated limb was similar to their respective mean contralateral control values; however, bilateral sciatic nerve stimulation at 1 Hz caused a less pronounced hyperaemic response in the muscles of the ligated limb, being 59, 63 and 49% of their mean control values in the gastrocnemius, plantaris and soleus muscles respectively. The rate of glucose utilization was determined by using the 2-deoxy3Hglucose method [Ferré, Leturque, Burnol, Penicaud furthermore, the rate of glucose utilization was significantly increased in the muscles of the ligated limb, being 140, 129 and 207% of their mean control values respectively. For the range of bloodflow to normally perfused skeletal muscle at rest or during isometric contraction determined in the present study, a linear correlation between the rate of glucose utilization and bloodflow can be demonstrated. Applying similar methods of regression analysis to glucose utilization and bloodflow to muscles of the ligated limb reveals a similar linear correlation. However, the rate of glucose utilization at a given bloodflow is increased in muscles of the ligated limb, indicating an adaptation of skeletal muscle to hypoperfusion.
Challiss et al. (Mon,) conducted a other in Arterial insufficiency. Unilateral femoral artery ligation and section vs. Contralateral control limb was evaluated on Rate of glucose utilization in gastrocnemius muscle at rest (µmol/min per 100g) (p=<0.05). Unilateral femoral artery ligation in rats significantly increased the rate of glucose utilization in the gastrocnemius and soleus muscles at rest to 126% and 140% of control values, respectively.