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The bilayer bending rigidities were measured for the same spherical vesicles with two methods, fluctuation mode analysis and electric deformation. In some of the sample cells the glue sealing the cell was in direct contact with the sample, in others the sample was shielded from the glue by a silicone grease. The values of the rigidity, while being lowest and practically equal for the unshielded samples, differed significantly between methods for the shielded ones. We also measured the temperature dependence of the bending rigidity. The dependence of the rigidity on method seems to suggest the existence of a membrane superstructure.
Niggemann et al. (Wed,) studied this question.