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1) Purification of JBE (Japanese B encephalitis) virus and morphological demonstration of the virus particles were tried by means of fluorocarbon treatment and elcetron microscopyFluorocarbon extract of brains of mice infected with JBE virus revealed numerous spherical particles, 60 mμ in diameter by the shadowing method of electron microscopyElectron microscopic observation on ultrathin sections of chick red cells which were precipitated in hemagglutination induced with the fluorocarbon extract revealed round particles 30 to 40 mμ in diameter with an obscure nucleoid, on the surface of the red cells.These particles were believed to be JBE virus.2) In vivo locailzation of JBE virus antigens was persued by fluorescent antiody method in the brains of mice infected with JBE virus. The antibody was prepared from the serum of rabbits treated previously with the fluorocarbon extract.Specific fluorescence which was seen in granular forms was observed in nerve cells and partially in neuroglia cells of the substantia nigra and thalamus.3) JBE virus antigens were observed by fluorescent antibody method in phagocytes present in the cerebrospinal fluid of patients with Japanese B encephalitis. This finding was believed to contribute to early diagnosis of Japanese B encephalitis.4) JBE virus antigens were demonstrated by fluorescent antibody method in nerve cells and partially neuroglia cells of the brain obtained by autopsy from patients with Japanese B encephalitis.Specific fluorescence was seen in the Purkinje cells in the cerebellum, and granular layer of the cerebral cortex and basal ganglia, particularly substantia nigra.
Kiyama et al. (Tue,) studied this question.