Studies of Gas and Electrolyte Equilibria in Blood

In a previous paper from this laboratory, Van Slyke, Hastings, and Neil1 (922) presented data quantitatively describing the effect of change in oxygenation on the COz dissociation curves and buffer values of horse blood. Recently we have required similar data for dog blood. However, the results of Van Slyke, Hastings, Heidelberger, and Neil1 (1922), with pure hemoglobin solutions and also those of Hastings, Van Slyke, Neill, Heidelberger, and Harington (1924), have indicated that the buffer values, and also the oxidation-reduction effects on the acidity of the hemoglobin, were quantitatively somewhat different in hemoglobins of the horse and dog. There was accordingly reason to expect similar differences in whole blood from the two species. We have therefore determined the CO2 dissociation curves and buffer values of oxygenated and reduced dog blood. The results are here presented, together with data for horse blood recalculated with the aid of blood constants which have been more accurately established since the work on horse blood was published. EXPERIMENTAL Technique of Saturation and AnalysisThe entire procedure was essentially the same as that used by Van Slyke, Hastings, and Neil1 (1922) in their Experiments 4, 5, and 6. With the exception of the blood for our Experiment 1, 0. 2 per cent potassium oxalate plus 0. 1 per cent sodium fluoride was