Introduction: is an important enzyme involved in the salvage of purine nucleotides. Materials & Methods: was carried out using two specific primers. The use of this fragment as a probe allowed the isolation of a larger cDNA sequence through searching the expressed sequence tag (EST) database. Results: . A band of 30.5 kDa was observed in the IPTG-induced sample compared to the control on SDS-PAGE. Protein expression was confirmed by Western blot analysis using an anti-His HRP-conjugated antibody. Conclusion: .
Jolodar Abbas (Mon,) studied this question.