Genetic inhibition of RyR2-S2814 phosphorylation prevented atrial fibrillation induction in FKBP12.6 knockout mice by suppressing sarcoplasmic reticulum calcium leak and delayed afterdepolarizations.
Does genetic inhibition of RyR2-S2814 phosphorylation prevent atrial fibrillation induction in FKBP12.6-deficient mice?
Genetic inhibition of RyR2-S2814 phosphorylation prevents atrial fibrillation induction in FKBP12.6 knockout mice by suppressing SR calcium leak and delayed afterdepolarizations, suggesting a potential therapeutic target.
RATIONALE: Abnormal calcium release from sarcoplasmic reticulum (SR) is considered an important trigger of atrial fibrillation (AF). Whereas increased Ca(2+)/calmodulin-dependent protein kinase II (CaMKII) activity has been proposed to contribute to SR leak and AF induction, downstream targets of CaMKII remain controversial. OBJECTIVE: To test the hypothesis that inhibition of CaMKII-phosphorylated type-2 ryanodine receptors (RyR2) prevents AF initiation in FKBP12.6-deficient (-/-) mice. METHODS AND RESULTS: Mice lacking RyR2-stabilizing subunit FKBP12.6 had a higher incidence of spontaneous and pacing-induced AF compared with wild-type mice. Atrial myocytes from FKBP12.6-/- mice exhibited spontaneous Ca(2+) waves (SCaWs) leading to Na(+)/Ca(2+)-exchanger activation and delayed afterdepolarizations (DADs). Mutation S2814A in RyR2, which inhibits CaMKII phosphorylation, reduced Ca(2+) spark frequency, SR Ca(2+) leak, and DADs in atrial myocytes from FKBP12.6-/-:S2814A mice compared with FKBP12.6-/- mice. Moreover, FKBP12.6-/-:S2814A mice exhibited a reduced susceptibility to inducible AF, whereas FKBP12.6-/-:S2808A mice were not protected from AF. CONCLUSIONS: FKBP12.6 mice exhibit AF caused by SR Ca(2+) leak, Na(+)/Ca(2+)-exchanger activation, and DADs, which promote triggered activity. Genetic inhibition of RyR2-S2814 phosphorylation prevents AF induction in FKBP12.6-/- mice by suppressing SR Ca(2+) leak and DADs. These results suggest suppression of RyR2-S2814 phosphorylation as a potential anti-AF therapeutic target.
Li et al. (Fri,) conducted a other in Atrial fibrillation. Mutation S2814A in RyR2 (genetic inhibition of CaMKII phosphorylation) vs. FKBP12.6-/- mice and wild-type mice was evaluated on Incidence of spontaneous and pacing-induced AF, SR Ca2+ leak, and delayed afterdepolarizations. Genetic inhibition of RyR2-S2814 phosphorylation prevented atrial fibrillation induction in FKBP12.6 knockout mice by suppressing sarcoplasmic reticulum calcium leak and delayed afterdepolarizations.