Analytical Mehod Development and Validation for the Determination of Aceclofenac and Rabeprazole by Uplc in Solid Dosage Forms

AbstractHPLC are reported analytical methods for compounds either individually or in combination withother dosage form. Method validation is the process to confirm that the analytical procedureemployed for a specific test is suitable for its intended use. Analytical testing of a pharmaceuticalproduct is necessary to ensure its purity, stability, safety and efficacy. Analytical methodvalidation is an integral part of the quality control system. The analytical method development andvalidation for the determination of aceclofenac and rabeprazole by UPLC in solid dosage formsand optimizing the chromatographic conditions. The developed method is validated according toICH guidelines for various parameters specified in ICH guidelines, Q2. Hence, it was felt that,there is a need of new analytical mehod development and validation for the determination ofaceclofenac and rabeprazole by UPLC in solid dosage forms. Retention time of Rabeprazole andAceclofenac were found 3.03 min and 1.84 min respectively. %RSD of the Rabeprazole andAceclofenac found 0.93 and 1.18 respectively. %Assay were obtained as 99.22% forRabeprazole and 99.65 % for Aceclofenac . Regression equation of Rabeprazole is y =3098.1578x + 45.2000. Regression equation of Aceclofenac is y=0.0002x-0.73090. Retentiontimes were decreased and that run time was decreased, so the method developed was simpleand economical that can be adopted in regular Quality control test in Industries.