DNA Chisel, a versatile sequence optimizer

MOTIVATION: Accounting for biological and practical requirements in DNA sequence design often results in challenging optimization problems. Current software solutions are problem-specific and hard to combine. RESULTS: DNA Chisel is an easy-to-use, easy-to-extend sequence optimization framework allowing to freely define and combine optimization specifications via Python scripts or Genbank annotations. AVAILABILITY AND IMPLEMENTATION: The framework is available as a web application (https: //cuba. genomefoundry. org/sculptₐₛequence) or open-source Python library (see at https: //github. com/Edinburgh-Genome-Foundry/DNAChisel for code and documentation). SUPPLEMENTARY INFORMATION: Supplementary data are available at Bioinformatics online.