Klebsiella pneumoniae is a major opportunistic pathogen responsible for a wide range of infections in both humans and animals. In this study, we report the draft genome sequence of K. pneumoniae strain KP-S-66, isolated from soil samples collected from the vicinity of a cattle shed. Whole-genome sequencing was performed using the Illumina NextSeq 2000 sequencing platform. The assembled draft genome comprises 5,175,725 bp distributed across 34 contigs, with an N 50 value of 371,700 bp. Genome annotation detected 32 antibiotic resistance genes (ARGs), with a predominance of genes involved in multidrug resistance (MDR) efflux pump mechanisms. In silico pathogenicity analysis showed a pathogenicity score of 0.98 (out of 1), indicating a high pathogenic potential despite its environmental origin. The draft genome sequence of strain KP-S-66 has been deposited in the National Center for Biotechnology Information (NCBI) GenBank under the accession numbers JBRZGN000000000, PRJNA1356658, SAMN53065372 and SRR35934007 for Genome, BioProject, BioSample and sequence read archive (SRA), respectively.
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