Asparagus cochinchinensis is a valued medicinal plant, whose bioactive polysaccharides are important secondary metabolites. However, the evolutionary origins and spatiotemporal regulation of their biosynthesis remain unclear, hindered by the lack of a reference genome. Here, we present the first chromosome-scale genome assembly of A. cochinchinensis (1.52 Gb; 10 pseudochromosomes), combining PacBio HiFi, Illumina, and Hi-C sequencing. We annotated 48,453 protein-coding genes and dated the divergence of Asparagus from Orchidaceae to ~95 million years ago. Comparative genomics revealed lineage-specific whole-genome duplication events and gene family expansions linked to metabolic adaptation. To resolve the spatial dynamics of polysaccharide biosynthesis, we generated transcriptomes from root tuber, fibrous root, leaf, and spear tissues. Co-expression network and pathway analyses identified 16 key genes significantly associated with polysaccharide accumulation, particularly in underground storage organs. Our analysis reconstructs the sucrose-to-polysaccharide biosynthetic pathway and highlights differential gene expression between tuber and fibrous root tissues, revealing the spatial compartmentalization of this metabolic process. This high-quality genome provides a foundational resource for elucidating the evolution and tissue-specific regulation of secondary metabolite biosynthesis in Asparagus, with implications for molecular breeding aimed at enhancing medicinal compound yield.
莫传鑫(Chuanxin Mo) (Thu,) studied this question.