Low-load resistance exercise with blood flow restriction increased p38MAPK phosphorylation at 2 hours (1.3 vs 0.4; p=0.05) and enhanced mRNA expression of VEGF and PGC-1α compared to control.
Does low-load resistance exercise with blood flow restriction improve angiogenic signalling in males?
Acute low-load resistance exercise with blood flow restriction provides a targeted angiogenic response, potentially mediated through enhanced ischaemic and shear stress stimuli.
Absolute Event Rate: 1.3% vs 0.4%
p-value: p=0.05
This study investigated protein kinase activation and gene expression of angiogenic factors in response to low-load resistance exercise with or without blood flow restriction (BFR). In a repeated measures cross-over design, six males performed four sets of bilateral knee extension exercise at 20% 1RM (reps per set = 30:15:15:continued to fatigue) with BFR (110 mmHg) and without (CON). Muscle biopsies were obtained from the vastus lateralis before, 2 and 4 h post-exercise. mRNA expression was determined using real-time RT-PCR. Protein phosphorylation/expression was determined using Western blot. p38MAPK phosphorylation was greater (p = 0.05) at 2 h following BFR (1.3 ± 0.8) compared to CON (0.4 ± 0.3). AMPK phosphorylation remained unchanged. PGC-1α mRNA expression increased at 2 h (5.9 ± 1.3 vs. 2.1 ± 0.8; p = 0.03) and 4 h (3.2 ± 0.8 vs. 1.5 ± 0.4; p = 0.03) following BFR exercise with no change in CON. PGC-1α protein expression did not change following either exercise. BFR exercise enhanced mRNA expression of vascular endothelial growth factor (VEGF) at 2 h (5.2 ± 2.8 vs 1.7 ± 1.1; p = .02) and 4 h (6.8 ± 4.9 vs. 2.5 ± 2.7; p = .01) compared to CON. mRNA expression of VEGF-R2 and hypoxia-inducible factor 1α increased following BFR exercise but only eNOS were enhanced relative to CON. Matrix metalloproteinase-9 mRNA expression was not altered in response to either exercise. Acute low-load resistance exercise with BFR provides a targeted angiogenic response potentially mediated through enhanced ischaemic and shear stress stimuli.
Ferguson et al. (Wed,) reported a other. Low-load resistance exercise with blood flow restriction vs. Low-load resistance exercise without blood flow restriction was evaluated on p38MAPK phosphorylation at 2 hours post-exercise (p=0.05). Low-load resistance exercise with blood flow restriction increased p38MAPK phosphorylation at 2 hours (1.3 vs 0.4; p=0.05) and enhanced mRNA expression of VEGF and PGC-1α compared to control.