In recent years, improvements in superovulation protocols and techniques and the determination of anti-Müllerian hormone (AMH) levels before superovulation applications in donor selection have increased the yield of transferable embryos obtained per unit time. The main objectives of this study were to compare the effect of two different follicular wave synchronization methods (GnRH and dominant follicle aspiration-DFA) applied on the same superovulation protocol timeline on superovulation response and to investigate the effect of superstimulation application on the level of AMH. A total of 30 cows were subjected to superovulation, consisting of two pre-synchronization (P) and one control (C) group, each containing 10 Holstein cows. Groups were formed as P-GnRH, P-DFA and C-GnRH. Blood samples were collected immediately before FSH injections (AMH-1) and 24 hours after superstimulation (AMH-2). The results showed that superovulation responses were higher in the presynchronized groups compared to the control (P0.05). There was no difference in AMH-1 levels among the groups. However, AMH-2 levels increased significantly in all groups (P0.01). In conclusion, it has been shown in cows that induction of ovulation of follicles with prolonged dominance by GnRH in the absence of CL or initiation of superstimulation after DFA results in similar embryo yields. In addition, circulating AMH levels are significantly increased after superstimulation treatments.
Mutlu et al. (Sun,) studied this question.
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