Rapid Diagnosis of Staphylococcus Aureus Using The Lamp Method

Methicillin-resistant Staphylococcus aureus (MRSA), a genetically distinct strain of S. aureus, poses a significant health problem in humans and animals. MRSA is responsible for various difficult-to-treat illnesses in humans and animals. The loop-mediated isothermal amplification (LAMP) experiment was performed using four unique primers developed for six distinct mecA and femA gene sequences. The LAMP test was completed in 45 minutes at 62.5°C and visualized by gel electrophoresis. Moreover, the LAMP test has better sensitivity than conventional PCR, detecting genomic DNA at 64.8 pg/μL, which is hundreds of times more sensitive than conventional PCR. The LAMP test is an attractive, potentially fascinating strategy for rapid detection of S. aureus that can be applied in laboratories and field settings with available budgets.