Separation of the hormone- and DNA-binding sites of the hepatic glucocorticoid receptor by means of proteolysis.

Chemistry, Karolinska Znstitutet, S- 104 01 Stockholm 60, Sweden The dexamethasone.receptor complex in rat liver cytosol has a Stokes radius of 61 A. It may be converted to smaller complexes with Stokes radii of 36 A and 19 A by proteolytic digestion with trypsin, a-chymotrypsin (that only gives rise to 36-A complex), papain, or an extract of the 1,000 x g to 10,000 x g pellet of liver homogenate. The 61-