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Progress in the nucleotide sequence analysis of sRNA has been very rapid in the last few years and the complete structure of alanine, serine, and tyrosine specific sRNAs has recently become known (Holley et al., 1965; Zachau, Dutting, and Feldmann, this volume; Madison, Everett and Kung, this volume). In this paper we shall report the data obtained so far on yeast phenylalanine sRNA, particularly the total number and nature of minor bases, the characterization of fragments produced by complete digestion with pancreatic RNase and T1-RNase, the construction of longer nucleotide sequence from the overlaps provided by minor bases or unique sequences in the above two sets of fragments, and the sequence of some larger oligonucleotides obtained by partial degradation with T1-RNase. From the total experimental information and a consideration of the strikingly similar structural features of sRNAs whose sequences are now established, a tentative total structure for yeast phenylalanine sRNA...
RajBhandary et al. (Sat,) studied this question.