Superimposed dyslipidaemia in diabetic mice increased vascular-ventricular stiffening and left ventricular myocardial fibrosis, and decreased cardiac output, compared to diabetes alone.
AIMS: Type II diabetes, often associated with abdominal obesity, frequently leads to heart failure. Clinical and epidemiological evidence suggests that supplemental dyslipidaemia and hypertension, as clustered in the metabolic syndrome, aggravate the cardiovascular outcome. The differential impact of type II diabetes and the metabolic syndrome on left ventricular function, however, remains incompletely defined. METHODS AND RESULTS: We studied left ventricular function in vivo using pressure-volume analysis in obese diabetic mice with leptin deficiency (ob/ob) and obese diabetic dyslipidemic mice with combined leptin and low-density lipoprotein-receptor deficiency (DKO). ob/ob and DKO mice developed a diabetic cardiomyopathy, characterized by impaired contractility and relaxation, from the age of 24 weeks onwards. This was-at least partially-explained by increased apoptosis and disturbed Ca(2+) reuptake in the sarcoplasmic reticulum (SR) in both mouse models. DKO, but not ob/ob, developed increased end-diastolic ventricular stiffness, paralleled by increased left ventricular myocardial fibrosis. Cardiac output was preserved in ob/ob mice by favourable loading conditions, whereas it decreased in DKO mice. CONCLUSIONS: Type II diabetes in mice leads to impaired contractility and relaxation due to disturbed Ca(2+) reuptake in the SR, but only when dyslipidaemia and hypertension are superimposed does vascular-ventricular stiffening increase and left ventricular myocardial fibrosis develop.
Bergh et al. (Tue,) conducted a other in Type II diabetes and metabolic syndrome. Combined leptin and low-density lipoprotein-receptor deficiency (DKO) / Dyslipidaemia vs. Obese diabetic mice with leptin deficiency (ob/ob) was evaluated on Left ventricular function (contractility, relaxation, ventricular stiffness, myocardial fibrosis, cardiac output). Superimposed dyslipidaemia in diabetic mice increased vascular-ventricular stiffening and left ventricular myocardial fibrosis, and decreased cardiac output, compared to diabetes alone.