Transgenic mouse hearts expressing slow skeletal troponin I showed a significantly blunted increase in Ca2+ sensitivity associated with increased sarcomere length compared to wild-type controls.
Expression of slow skeletal troponin I in mouse hearts blunts the length-dependent increase in Ca2+ sensitivity of myofilaments, providing a basis for testing the significance of length dependence of activation in situ.
We compared sarcomere length (SL) dependence of the Ca2+-force relation of detergent-extracted bundles of fibres dissected from the left ventricle of wild-type (WT) and transgenic mouse hearts expressing slow skeletal troponin I (ssTnI-TG). Fibre bundles from the hearts of the ssTnI-TG demonstrated a complete replacement of the cardiac troponin I (cTnI) by ssTnI. Compared to WT controls, ssTnI-TG fibre bundles were more sensitive to Ca2+ at both short SL (1.9 +/- 0.1 micrometer) and long SL (2.3 +/- 0.1 micrometer). However, compared to WT controls, the increase in Ca2+ sensitivity (change in half-maximally activating free Ca2+; DeltaEC50) associated with the increase in SL was significantly blunted in the ssTnI-TG myofilaments. Agents that sensitize the myofilaments to Ca2+ by promoting the actin-myosin reaction (EMD 57033 and CGP-48506) significantly reduced the length-dependent DeltaEC50 for Ca2+ activation, when SL in WT myofilaments was increased from 1.9 to 2.3 micrometer. Exposure of myofilaments to calmidazolium (CDZ), which binds to cTnC and increases its affinity for Ca2+, sensitized force developed by WT myofilaments to Ca2+ at SL 1.9 micrometer and desensitized the WT myofilaments at SL 2.3 micrometer. There were no significant effects of CDZ on ssTnI-TG myofilaments at either SL. Our results indicate that length-dependent Ca2+ activation is modified by specific changes in thin filament proteins and by agents that promote the actin-myosin interaction. Thus, these in vitro results provide a basis for using these models to test the relative significance of the length dependence of activation in situ.
Arteaga et al. (Tue,) reported a other. Expression of slow skeletal troponin I (ssTnI-TG) vs. Wild-type (WT) controls was evaluated on Sarcomere length dependence of the Ca2+-force relation (change in half-maximally activating free Ca2+; DeltaEC50). Transgenic mouse hearts expressing slow skeletal troponin I showed a significantly blunted increase in Ca2+ sensitivity associated with increased sarcomere length compared to wild-type controls.