FIGURE 4 from Spectral Flow Cytometry Methods and Pipelines for Comprehensive Immunoprofiling of Human Peripheral Blood and Bone Marrow

Cell relationships and marker complexity. A, PBMC cell relationship schematic. B, BMC cell relationship schematic. Solid lines in A and B reflect canonically gated cell populations, while dashed lines represent intermediate gating steps. C–E, Clustered heatmaps displaying relative expression of different markers across defined cell types depicted in A and B. Columns represents the expression of each marker while rows indicate the defined cell types. The marker mean fluorescence intensity is displayed on a scale from white (lowest) to green (highest).