The Development and Validation of a Simple HPLC-UV Method for the Determination of Vancomycin Concentration in Human Plasma and Application in Critically Ill Patients

> 0.99). Inter- and intra-day assay precision and accuracy were determined to be within the acceptable range. The run time was noted to be 10 min. This method was evaluated using different greenness tools, which indicated that the method is environmentally friendly. Our method was effectively applied to analyze vancomycin concentrations in critically ill patients. Thus, our approach has the potential for practical implementation in routine TDM procedures.