XPO1 is overexpressed in colorectal cancer. A, Boxplot derived from Gene Expression Profiling Interactive Analysis showing colon adenocarcinoma (COAD) tissue compared with normal colon tissue. (The Cancer Genome Atlas data derived from the online website Gene Expression Profiling Interactive Analysis: gepia.cancer-pku.cnn/detail.php?gene=XPO1). Based on a one-way ANOVA statistical test, the change in XPO1 expression is not significant. B, XPO1 mRNA expression by qPCR in major colorectal cancer tumor stages normalized to normal colon tissue. Actin was used as the loading control, and values represent mean fold change ± SEM. For normal human colon tissue, n = 8; for stage I colorectal cancer tissue, n = 5; for stage II colorectal cancer tissue, n = 9; for stage III colorectal cancer tissue, n = 16; and for stage IV colorectal cancer tissue, n = 10. A Student t test was used to statistically compare each colorectal cancer–staged tissue group to the normal colon tissue group. C, XPO1 mRNA expression by qPCR in multiple colorectal cancer cell lines compared with normal colon tissue. GAPDH was used as the loading control, and values represent mean fold change ± SEM. A Student t test was used to statistically compare each cell line to normal human colon tissue group. D, Eltanexor structure and XPO1 structure highlighting eltanexor’s protein docking region in XPO1’s protein-binding groove. (PDB: 6XJT; *, P ≤ 0.05; ***, P ≤ 0.001; ****, P ≤ 0.0001). CRC, colorectal cancer.
Evans et al. (Tue,) studied this question.