This protocol describes the manual extraction and SPRI of HMW DNA from fresh frozen jellyfish samples intended for long-read sequencing, using a combination of the Omega Bio-Tek E.Z.N.A.® Mollusc it was primarily adapted from the Omega Bio-Tek E.Z.N.A.® Mollusc & Insect DNA kit protocol by combining the initial lysis and precipitation (steps 1 to 10) with reduced incubation temperatures for jellyfish samples, omission of vortexing steps and the replacement of the spin-columns with a bead-based extraction using Sera-Mag™ SpeedBeads, as utilised in the Sanger Tree of Life HMW DNA Extraction: Automated Plant Organic HMW gDNA Extraction (POE) protocol. Acronyms HMW: high molecular weight LI: low input ULI: ultra-low input
Denton et al. (Tue,) studied this question.