T1‐weighted fMRI in mouse visual cortex using an iron oxide nanoparticle contrast agent and UTE imaging at 9.4 T

Abstract Purpose This study aims to investigate the feasibility of using T 1 ‐weighted fMRI with an iron oxide nanoparticle contrast agent and UTE imaging at 9.4 T to measure functional hyperemia in the mouse visual cortex. The goal is to capture positive signal changes in both the parenchyma and pial surface, and to test whether surface vessels respond during neuronal activation. Method The study involved scanning of nine mice after administration of iron oxide–based superparamagnetic contrast agent via the tail vein. Two functional imaging experiments were conducted: one to investigate the effect of TE on the functional response, and another to characterize the impact of higher resolution on UTE functional contrast. Regions of interest were defined in the parenchyma and pial surface of the visual cortex. Results The administration of the contrast agent produced a bright‐blood signal in the vasculature in structural MRI when using a UTE acquisition. Positive signal changes were observed at the shortest TE (0.164 ms) in voxels sampling both the parenchyma (0.22% ± 0.08%) and pial surface (0.26% ± 0.1%), providing evidence that UTE fMRI experiments can detect changes in both parenchymal and pial vessels. Measurements using longer TEs (≥1 ms) showed negative signal changes, as expected. Higher spatial resolution resulted in increased percent signal change at the pial surface, suggesting reduced partial volume effects. Conclusion The findings demonstrate that T 1 ‐weighted fMRI with UTE imaging and Superparamagnetic Iron Oxide nanoparticles captures positive signal changes across all vascular compartments, providing additional insights into the involvement of surface vessels during functional hyperemia.