Bio-Detection Dogs Sniff COVID-19 in Thailand

firstₚage Download PDF settings Order Article Reprints Font Type: Arial Georgia Verdana Font Size: Aa Aa Aa Line Spacing:    Column Width:    Background: Open AccessAbstract Bio-Detection Dogs Sniff COVID-19 in Thailand † by Isaya ThaveesangsakulthaiIsaya Thaveesangsakulthai SciProfiles Scilit Preprints. org Google Scholar 1, *, Sorrawit SongsathitmethaSorrawit Songsathitmetha SciProfiles Scilit Preprints. org Google Scholar 2, Chadin KulsingChadin Kulsing SciProfiles Scilit Preprints. org Google Scholar 1 and Kaywalee ChatdarongKaywalee Chatdarong SciProfiles Scilit Preprints. org Google Scholar 3 1 Department of Chemistry, Faculty of Science, Chulalongkorn University, Bangkok 10330, Thailand 2 Department of Pathology, Faculty of Veterinary Science, Chulalongkorn University, Bangkok 10330, Thailand 3 Department of Obstetrics Gynaecology and Reproduction, Faculty of Veterinary Science, Chulalongkorn University, Bangkok 10330, Thailand * Author to whom correspondence should be addressed. † Presented at the 4th International Electronic Conference on Biosensors, 20–22 May 2024; Available online: https: //sciforum. net/event/IECB2024. Proceedings 2024, 104 (1), 32; https: //doi. org/10. 3390/proceedings2024104032 Published: 28 May 2024 (This article belongs to the Proceedings of The 4th International Electronic Conference on Biosensors) Download keyboardₐrrowdown Download PDF Download PDF with Cover Download XML Download Epub Versions Notes Keywords: SARS-CoV-2; bio detection dogs; VOCs; screening test; non-invasive Severe acute respiratory syndrome coronavirus (SARS-CoV-2) caused a pandemic COVID-19 disease worldwide, generating an urgent need to develop an early diagnosis approach. This work applied trained dogs to perform a sniff test for COVID-19 screening based on the detection of volatile organic compound (VOC) markers in sweat. Male and female Labrador Retrievers participated in this research as sniffer dogs. The samples were collected from the armpit sweat of PCR-positive (symptomatic/asymptomatic) and -negative patients admitted at Chulalongkorn University Hospital, Bangkok, Thailand. Two cotton rods were used to collect the samples from each patient and kept in 20 mL screw headspace glass vials closed with aluminum caps. The virus inactivation on the surface was performed under UV radiation and left at 25 °C inside a biosafety laboratory level 2 (BSL2) at Chulalongkorn University Hospital for 48 h. The cotton swabs from each patient were transferred from the glass vial into a stainless steel bottle with dimensions of 5 × 9. 5 cm. The dog training process was based on positive reinforcement approaches, involving memorizing the COVID-19 positive and negative samples which were hanging in an I-shaped stand. A COVID-19 screening test evaluation (accuracy, sensitivity, and specificity) was carried out using a six-armed spinning wheel. The sniffer dogs successfully recognized VOC markers for SARS-CoV-2 patients with a success rate of 96% (n = 300). In conclusion, the COVID-19 sniffer dogs have the capability for participating in COVID-19 point-of-care testing in public areas in Bangkok, Thailand. Author ContributionsConceptualization, I. T. and K. C. ; methodology, I. T. and K. C. , software, S. S. ; validation, I. T. , K. C. and S. S. ; formal analysis, C. K. ; investigation, S. S. , K. C. and C. K. ; resources, S. S. , K. C. and C. K. ; data curation, I. T. ; writing—original draft preparation, I. T. and S. S. ; writing—review and editing, I. T. and S. S. ; visualization, K. C. and C. K. ; supervision, S. S. and K. C. ; funding acquisition, K. C. All authors have read and agreed to the published version of the manuscript. FundingThis research was funded by Chevron Thailand Exploration and Production, Ltd. Institutional Review Board StatementThe study was conducted in accordance with the Declaration of Central Research Ethics Committee (COA-CREC103/2020), and approved by the Institutional Review Board at the Faculty of Medicine, Chulalongkorn University (897/63). Informed Consent StatementInformed consent was obtained from all subjects involved in the study. Data Availability StatementThe data that support the findings of this study are available on request from the corresponding author. The data are not publicly available due to privacy or ethical restrictions. Conflicts of InterestThe authors declare that this study received funding from Chevron Thailand Exploration and Production, Ltd. The funder had the following involvement with the study: K9 dogs sniff COVID-19 in Thailand. The authors declare that this study received funding from Chevron Thailand Exploration and Production, Ltd. The funder was not involved in the study design, collection, analysis, interpretation of data, the writing of this article or the decision to submit it for publication. Disclaimer/Publisher's Note: The statements, opinions and data contained in all publications are solely those of the individual author (s) and contributor (s) and not of MDPI and/or the editor (s). MDPI and/or the editor (s) disclaim responsibility for any injury to people or property resulting from any ideas, methods, instructions or products referred to in the content. © 2024 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https: //creativecommons. org/licenses/by/4. 0/). Share and Cite MDPI and ACS Style Thaveesangsakulthai, I. ; Songsathitmetha, S. ; Kulsing, C. ; Chatdarong, K. Bio-Detection Dogs Sniff COVID-19 in Thailand. Proceedings 2024, 104, 32. https: //doi. org/10. 3390/proceedings2024104032 AMA Style Thaveesangsakulthai I, Songsathitmetha S, Kulsing C, Chatdarong K. Bio-Detection Dogs Sniff COVID-19 in Thailand. Proceedings. 2024; 104 (1): 32. https: //doi. org/10. 3390/proceedings2024104032 Chicago/Turabian Style Thaveesangsakulthai, Isaya, Sorrawit Songsathitmetha, Chadin Kulsing, and Kaywalee Chatdarong. 2024. "Bio-Detection Dogs Sniff COVID-19 in Thailand" Proceedings 104, no. 1: 32. https: //doi. org/10. 3390/proceedings2024104032 Article Metrics No No Article Access Statistics Multiple requests from the same IP address are counted as one view.