The Development and Standardization of a U-Bent LSPR Fiber Optic Biosensor to Screen for Parvovirus B19 IgM

firstₚage Download PDF settings Order Article Reprints Font Type: Arial Georgia Verdana Font Size: Aa Aa Aa Line Spacing:    Column Width:    Background: Open AccessAbstract The Development and Standardization of a U-Bent LSPR Fiber Optic Biosensor to Screen for Parvovirus B19 IgM † by Reuben Kuruvilla ThomasReuben Kuruvilla Thomas SciProfiles Scilit Preprints. org Google Scholar 1, *, Ratan Kumar ChaudharyRatan Kumar Chaudhary SciProfiles Scilit Preprints. org Google Scholar 2, Vikraman AruldossVikraman Aruldoss SciProfiles Scilit Preprints. org Google Scholar 2, Swati KumariSwati Kumari SciProfiles Scilit Preprints. org Google Scholar 1, Padma SrikanthPadma Srikanth SciProfiles Scilit Preprints. org Google Scholar 1 and V. V. Raghavendra SaiV. V. Raghavendra Sai SciProfiles Scilit Preprints. org Google Scholar 2 1 Department of Microbiology, Sri Ramachandra Institute of Higher Education and Research, Chennai 600116, India 2 Department of Applied Mechanics, Indian Institute of Technology (IITM), Chennai 600036, India * Author to whom correspondence should be addressed. † Presented at the 4th International Electronic Conference on Biosensors, 20–22 May 2024; Available online: https: //sciforum. net/event/IECB2024. Proceedings 2024, 104 (1), 20; https: //doi. org/10. 3390/proceedings2024104020 Published: 28 May 2024 (This article belongs to the Proceedings of The 4th International Electronic Conference on Biosensors) Download keyboardₐrrowdown Download PDF Download PDF with Cover Download XML Download Epub Versions Notes Keywords: parvovirus B19; LSPR; biosensor; fiber optic Introduction. Nearly 50% of the adult population has been exposed to parvovirus B19 (B19V). B19V has an elevated risk of transmission through blood and blood products as it is resistant to common inactivation procedures. Multiple studies have documented high IgM seroprevalence among blood donors that were recently exposed to B19V, which could pose a risk to blood safety programs. This study aimed to develop a lateral surface plasmon resonance (LSPR) U-Bent fiber optic biosensor (fob) that could screen B19V IgM. Methods. A linear epitope against B19V was identified using Bepipred2. 0 and commercially synthesized. The epitope was validated using ELISA against synthetic IgM control and then was tested against clinical B19V IgM samples. A label-free method was employed to mount the biotinylated epitope on the LSPR fob and then test the absorbance against B19V IgM. A bump in the spectral absorbance curve indicated that the sample was positive for B19V IgM. The fob was calibrated at each step, measuring the pre- and post-wash absorbance reading to accommodate the different buffers. Results. The epitope demonstrated suitable reactivity against B19V IgM as demonstrated by ELISA. The label-free method was suitable in the identification of low levels of a synthetic B19V antibody with similar detection indices to B19V ELISA. Discussion. Individuals who screen as B19V IgM-positive (that indicates recent infection) can then be screened for B19V DNA. The transfusion of B19V DNA-positive blood and blood products can lead to severe consequences for individuals who are hematologically stressed or immunocompromised. Multiple studies have documented the importance of screening for B19V especially among blood donors by the introduction of routine screening into their national blood safety programs. A sensitive and specific rapid test such as this LSPR fob would be very beneficial in the B19V screening. Author ContributionsConceptualization, R. K. T. , P. S. and V. V. R. S. ; methodology, V. A. , R. K. C. and R. K. T. ; software, R. K. T. , V. A. and R. K. C. ; validation, R. K. T. , S. K. , V. A. and R. K. C. ; formal analysis, V. V. R. S. and R. K. C. ; resources, P. S. and V. V. R. S. ; writing—original draft preparation, R. K. T. ; writing—review and editing, R. K. T. ; visualization, R. K. T. , V. A. and R. K. C. ; supervision, V. V. R. S. and P. S. ; project administration, R. K. T. , P. S. and V. V. R. S. ; funding acquisition, R. K. T. and P. S. All authors have read and agreed to the published version of the manuscript. FundingScheme for the Promotion of Academic and Research Collaboration (SPARC), Ministry of Human Resource Development, Govt. of India. Institutional Review Board StatementEthics approval was received from Sri Ramachandra Institute of Higher Education and Research (IEC – NI/19/FEB/68/08). Informed Consent StatementNot applicable. Data Availability StatementAll data supporting the findings in this study is available with the corresponding author. Conflicts of InterestThe authors declare no conflict of interest. Disclaimer/Publisher's Note: The statements, opinions and data contained in all publications are solely those of the individual author (s) and contributor (s) and not of MDPI and/or the editor (s). MDPI and/or the editor (s) disclaim responsibility for any injury to people or property resulting from any ideas, methods, instructions or products referred to in the content. © 2024 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https: //creativecommons. org/licenses/by/4. 0/). Share and Cite MDPI and ACS Style Kuruvilla Thomas, R. ; Chaudhary, R. K. ; Aruldoss, V. ; Kumari, S. ; Srikanth, P. ; Sai, V. V. R. The Development and Standardization of a U-Bent LSPR Fiber Optic Biosensor to Screen for Parvovirus B19 IgM. Proceedings 2024, 104, 20. https: //doi. org/10. 3390/proceedings2024104020 AMA Style Kuruvilla Thomas R, Chaudhary RK, Aruldoss V, Kumari S, Srikanth P, Sai VVR. The Development and Standardization of a U-Bent LSPR Fiber Optic Biosensor to Screen for Parvovirus B19 IgM. Proceedings. 2024; 104 (1): 20. https: //doi. org/10. 3390/proceedings2024104020 Chicago/Turabian Style Kuruvilla Thomas, Reuben, Ratan Kumar Chaudhary, Vikraman Aruldoss, Swati Kumari, Padma Srikanth, and V. V. Raghavendra Sai. 2024. "The Development and Standardization of a U-Bent LSPR Fiber Optic Biosensor to Screen for Parvovirus B19 IgM" Proceedings 104, no. 1: 20. https: //doi. org/10. 3390/proceedings2024104020 Article Metrics No No Article Access Statistics Multiple requests from the same IP address are counted as one view.