Development of Covalent Inhibitors for Bacterial Histidine Kinases

Key Points

• To develop covalent inhibitors targeting bacterial histidine kinases as a novel treatment strategy against antimicrobial resistance.
• Synthesized 2-aminobenzothiazole sulfonyl fluoride analogs
• Conducted in vitro assays to evaluate inhibitor efficacy
• Performed in cellulo assays for effectiveness in biological systems
• Identified strong lead inhibitors for bacterial histidine kinases
• Validated the potential of antivirulence therapies against antimicrobial resistance
• Demonstrated successful inhibition of kinases in vitro and in cellulo