Integrated Strategy for Breast Cancer Biomarker Analysis Using Dual Ionic Liquid Aqueous Biphasic Systems and Microfluidic Immunoassays

Key Points

• The study aims to improve breast cancer biomarker analysis by utilizing dual ionic liquid aqueous biphasic systems and microfluidic immunoassays.
• Developed a dual ionic liquid aqueous biphasic system for biomarker extraction.
• Implemented microfluidic immunoassays for analyzing HER2 levels.
• Conducted serum-based testing as an alternative to tissue biopsy.
• Successfully detected HER2 levels in serum samples using the new method.
• Showed that the dual ionic liquid system enhances biomarker recovery and specificity.
• Indicated potential for faster and more accurate breast cancer diagnosis.