Identification of the Novel HLA‐B*58:162 Allele by Next‐Generation Sequencing

Key Points

• The research aims to identify a novel allele variant of HLA-B through sequencing techniques.
• Utilized next-generation sequencing for genetic analysis
• Focused on HLA-B alleles and nucleotide variations
• Analyzed nucleotide changes specifically at codon 66 in exon 2
• Discovered HLA-B*58:162 as a new allele variant
• Identified a nucleotide change from HLA-B*58:01:01:01 at codon 66
• Confirmed the significance of the nucleotide change in the allele's identification