What question did this study set out to answer?

This research aims to evaluate the sensitivity and robustness of a one-pot assay for detecting Avian Leukosis Virus subgroup J (ALV-J).

May 3, 2026

Sensitive and Robust One-Pot RPA-CRISPR/Cas12a Assay with Elimination of cis-Cleavage.

Key Points

This research aims to evaluate the sensitivity and robustness of a one-pot assay for detecting Avian Leukosis Virus subgroup J (ALV-J).
Developed a one-pot assay combining RPA with CRISPR/Cas12a technology.
Tested the assay's performance using ALV-J samples.
Focused on eliminating cis-cleavage to improve assay effectiveness.
The 11bp+9ss one-pot assay showed superior sensitivity compared to existing methods.
Exhibited robust performance, indicating its potential for point-of-care testing (POCT) for bacteria and viruses.

Abstract

and Avian Leukosis Virus subgroup J (ALV-J) samples. Overall, the 11bp+9ss one-pot assay exhibits superior sensitivity and robustness, showing great potential for POCT of bacteria and viruses.

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