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May 1, 2026

Sensitive and Robust One-Pot RPA-CRISPR/Cas12a Assay with Elimination of cis-Cleavage.

Ruiying Han Ning Xiao Zhirui Wu Yingying Zhao X Wang Xiaochuan Tang

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Key Points

This research aims to evaluate the sensitivity and robustness of a one-pot assay for detecting Avian Leukosis Virus subgroup J (ALV-J).
Developed a one-pot assay combining RPA with CRISPR/Cas12a technology.
Tested the assay's performance using ALV-J samples.
Focused on eliminating cis-cleavage to improve assay effectiveness.
The 11bp+9ss one-pot assay showed superior sensitivity compared to existing methods.
Exhibited robust performance, indicating its potential for point-of-care testing (POCT) for bacteria and viruses.

Abstract

and Avian Leukosis Virus subgroup J (ALV-J) samples. Overall, the 11bp+9ss one-pot assay exhibits superior sensitivity and robustness, showing great potential for POCT of bacteria and viruses.

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Cite This Study

Han et al. (2026) studied this question.

synapsesocial.com/papers/69f6e6e68071d4f1bdfc7802 https://doi.org/10.1021/acs.analchem.5c06674

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Keep reading

Synapse has enriched 5 closely related papers on similar clinical questions. Consider them for comparative context:

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5crRNA Truncation Minimizes <i>cis</i> -Cleavage for Universal One-Pot RPA-CRISPR-Cas12a Detection with Attomolar SensitivityOriginal Research · 2026 · 5 citations