Sinefungin Biosynthesis Employs a B 12 -Dependent Radical S -Adenosyl- l -methionine Enzyme Catalyzing C-Adenosylation of Arginine

Key Points

• The aim is to explore the biosynthesis of sinefungin and identify the enzymatic processes involved.
• Investigation of the radical SAM enzyme family
• Characterization of the enzyme's activity
• Analysis of the assembly pathway for conjugates
• Identification of a novel B12-dependent radical SAM enzyme
• Revealed unique mechanisms for C-adenosylation of arginine
• Demonstrated unconventional pathways for amino acid-nucleoside conjugate synthesis