Study on the mechanism of italicBushen Huoxue Formula/italic （补肾活血方） in regulating Th17 cell differentiation bias for the treatment of unexplained recurrent spontaneous abortion

Key Points

• The aim is to explore how Bushen Huoxue Formula regulates Th17 cell differentiation to treat unexplained recurrent spontaneous abortion (URSA).
• Constructed normal and URSA pregnancy mouse models using CBA/J and DBA/2 mice.
• Divided URSA mice into model, Chinese medicine, and Western medicine groups, with 15 mice each.
• Administered treatments and monitored embryo absorption rates and protein/mRNA expression levels.
• URSA group showed significantly increased embryo absorption rates (P<0.05) and elevated serum levels of IL-17 (P<0.05) and IL-22 (P<0.01).
• Chinese medicine group significantly reduced embryo absorption rates (P<0.05) and downregulated RORγt, IL-17, and IL-22 levels (P<0.01) compared to model group.
• No significant difference in outcomes between Chinese and Western medicine groups (P>0.05).