Simplifications in the synthesis of short oligonucleotide blocks

Key Points

• The aim is to develop a rapid synthesis method for short oligonucleotide blocks.
• A new procedure utilizing aryl phosphoroditriazolide was developed.
• The method allows direct synthesis of mono, di, and trideoxyribonucleotides.
• Trinucleotide blocks are synthesized without purification at the dimer stage.
• The new method is faster and more convenient compared to traditional coupling strategies.
• It minimizes drawbacks associated with bifunctional phosphorylating reagents.
• Fully protected nucleotides were successfully synthesized with higher efficiency.