Adenosine exerted an anti-adrenergic effect on isolated ventricular myocytes that was most pronounced in failing human hearts and was abolished by pertussis toxin pretreatment.
Adenosine exerts a pronounced anti-adrenergic effect in failing human heart myocytes, mediated by A1 receptors coupled to Gi/Go proteins.
In intact ventricular preparation, adenosine has been shown to reduce the beta-adrenoceptor-induced increase in contraction (the anti-adrenergic effect). In the present study we have investigated this effect of adenosine on isolated ventricular myocytes from failing human heart and normal guinea-pig and rat heart. 2. Adenosine in the absence of beta-adrenoceptor-mediated stimulation had no effect on contraction in human and guinea-pig myocytes but produced a variable effect in rat myocytes. 3. 8-Cyclopentyl 1,3-dipropylxanthine (CPX), a selective A1-receptor antagonist, antagonised the anti-adrenergic effect of adenosine in guinea-pig myocytes. 4. The anti-adrenergic effect of adenosine was greater in guinea-pig than rat myocytes and even more pronounced in cells isolated from failing human heart. 5. Pertussis toxin-pretreatment at 35 degrees C of guinea-pig and human myocytes abolished the anti-adrenergic effect of adenosine. Longer exposure to higher concentrations of pertussis toxin was required for complete abolition in human compared to guinea-pig cells. 6. These results support the suggestion that the adenosine receptors mediating the anti-adrenergic effect of adenosine are of the A1 subtype and are coupled to the inhibitory guanine nucleotide binding protein, Gi/Go. 7. Pertussis toxin pretreatment increased the sensitivity of guinea-pig myocytes to isoprenaline in the absence of adenosine; the EC50 value was decreased by a factor of 10. This suggests that Gi may exert a tonic inhibitory effect on the beta-adrenoceptor/adenylate cyclase interaction in normal myocardium.
Brown et al. (Mon,) conducted a other in Failing human heart, normal guinea-pig and rat heart. Adenosine and pertussis toxin was evaluated on Anti-adrenergic effect (reduction in beta-adrenoceptor-induced increase in contraction). Adenosine exerted an anti-adrenergic effect on isolated ventricular myocytes that was most pronounced in failing human hearts and was abolished by pertussis toxin pretreatment.