Three-dimensional reconstruction of mouse ventricular walls at the micrometer scale revealed a cardiomyocyte arrangement aligned to the long-axis direction of the outer ventricular walls.
The study provides the first micrometer-scale 3D reconstruction of cardiomyocyte orientation in mouse hearts, revealing a long-axis fiber continuum that can inform future studies on heart mechanics and disease remodeling.
Coordinated cardiomyocyte contraction drives the mammalian heart to beat and circulate blood. No consensus model of cardiomyocyte geometrical arrangement exists, due to the limited spatial resolution of whole heart imaging methods and the piecemeal nature of studies based on histological sections. By combining microscopy and computer vision, we produced the first-ever three-dimensional cardiomyocyte orientation reconstruction across mouse ventricular walls at the micrometer scale, representing a gain of three orders of magnitude in spatial resolution. We recovered a cardiomyocyte arrangement aligned to the long-axis direction of the outer ventricular walls. This cellular network lies in a thin shell and forms a continuum with longitudinally arranged cardiomyocytes in the inner walls, with a complex geometry at the apex. Our reconstruction methods can be applied at fine spatial scales to further understanding of heart wall electrical function and mechanics, and set the stage for the study of micron-scale fiber remodeling in heart disease.
Dileep et al. (Wed,) reported a other. Microscopy and computer vision 3D reconstruction was evaluated on 3D cardiomyocyte orientation. Three-dimensional reconstruction of mouse ventricular walls at the micrometer scale revealed a cardiomyocyte arrangement aligned to the long-axis direction of the outer ventricular walls.
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