Extended volume confocal microscopy revealed that perimysial collagen in the rat left ventricle has an ordered laminar arrangement in the midwall and subendocardium, but forms longitudinal cords in the subepicardium.
Observational (n=7)
No
p-value: p=0.002
There is strong support for the view that the ventricular myocardium has a laminar organization in which myocytes are grouped into branching layers separated by cleavage planes. However, understanding of the extent and functional implications of this architecture has been limited by the lack of a systematic three-dimensional description of the organization of myocytes and associated perimysial collagen. We imaged myocytes and collagen across the left ventricular wall at high resolution in seven normal rat hearts using extended volume confocal microscopy. We developed novel reconstruction and segmentation techniques necessary for the quantitative analysis of three-dimensional myocyte and perimysial collagen organization. The results confirm that perimysial collagen has an ordered arrangement and that it defines a laminar organization. Perimysial collagen is composed of three distinct forms: extensive meshwork on laminar surfaces, convoluted fibers connecting adjacent layers, and longitudinal cords. While myolaminae are the principal form of structural organization throughout most of the wall, they are not seen in the subepicardium, where perimysial collagen is present only as longitudinal cords.
Pope et al. (Sat,) conducted a observational in Normal cardiac anatomy (n=7). Extended volume confocal microscopy was evaluated on Normalized variance of collagen colinearity across transmural regions (p=0.002). Extended volume confocal microscopy revealed that perimysial collagen in the rat left ventricle has an ordered laminar arrangement in the midwall and subendocardium, but forms longitudinal cords in the subepicardium.