Fallopian tube alters sEVs protein cargo after treatment with OVCAR3 EVs for 14 days. A, Diagram showing EV treatment of hFTE tissue and production of secondary-release EVs. B, Volcano plot showing proteomic changes in EVs derived from tissues treated with OVCAR3 EVs compared with PBS control. C, Volcano plot showing proteomics changes in EVs derived from tissues treated with FT240 EVs compared with PBS-treated controls. D, Venn diagram showing identification of 25 proteins exclusively upregulated by OVCAR3 EVs only. E, Venn diagram showing detection of DEPs not present in original OVCAR3 EVs in hFTE-derived EVs. F, GO biological pathway analysis of DEPs in hFTE-derived secondary-release EVs following stimulation with OVCAR3 EVs.
Sipes et al. (Mon,) studied this question.