Abstract The tomato leaf curl New Delhi virus (ToLCNDV), also known as Begomovirus solanumdelhiense is a whitefly (Bemisia tabaci) -transmitted bipartite begomovirus that causes significant economic losses exceeding USD 300 million annually. It has been reported to infect a wide range of commercially important crops such as tomato, papaya, golden melon, watermelon and luffa. The ToLCNDV genome consists of two components, DNA-A and DNA-B. In this study, we confirmed the presence of viral genome from the gDNA extracted from a symptomatic golden melon plant in Sarawak, Malaysia. The complete bipartite genome was recovered using Illumina shotgun metagenomic approach, offering a primer-free alternative to conventional Sanger-based methods, enabling detection of more divergent viral variants. Comparative genomics against publicly available ToLCNDV genomes from public database showed that the Sarawak strain clusters closely with other Southeast Asian ToLCNDV strains. Notably, the DNA-B segment demonstrated greater discriminatory power for grouping isolates by geographic origin rather than host specificity.
Chong et al. (Thu,) studied this question.
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