Although alkaloids are physiologically active, their toxic effects on organ systems and genetic toxicity risks cannot be ignored, necessitating rapid and simultaneous detection of these hazardous compounds. Using tetrahydro-1H-pyrrolizine-7a(5H)-acetic acid hydrochloride as the hapten, we developed a group-specific monoclonal antibody (mAb 1B6) capable of simultaneously recognizing nine alkaloids (senecionine, retrorsine, seneciphylline, monocrotaline, usaramine, atropine, l-hyoscyamine, scopolamine, and physostigmine). The three-dimensional structure of mAb 1B6 was successfully constructed by homology modeling to study the antibody recognition mechanism. Based on this antibody, a gold nanoparticle-based immunochromatographic strip (AuNP-ICS) was established for detecting these alkaloids in wheat, with visual detection limits of 100-1000 μg/kg and calculated detection limits of 5.84-100.53 μg/kg. Spiked recovery experiments confirmed that the method was accurate and stable, and yielded recovery rates of 95.39%-105.76% and coefficients of variation of 1.02%-3.22%, consistent with LC-MS/MS results. Therefore, this AuNP-ICS represents a practical tool for rapidly detecting alkaloids in wheat.
Li et al. (Mon,) studied this question.
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