BACKGROUND: Listeria infection triggers the production of ROS (Reactive Oxygen Species) and radical-waste accumulation such as Malondialdehyde (MDA). Andrographis paniculata, triggers the production of Superoxide Dismutase (SOD) as antioxidant and suppresses the bacteria multiplication in host’s body. However, these active-phytochemical have poor destruction due to their large size and molecular weight. Nanoparticle technology may overcome this problem to increase bioavailability and benefit of herbal pharmacology. AIMS: to evaluate the effectivity nanoparticle of A. paniculata extract to increase bacteria elimination, increase SOD level, and reduce MDA level in rat infected with L. monocytogenes METHOD: Experimental research with post-test only controlled trial design was conducted using white rats (Rattus novergicus strain Wistar) into Normal (N), Negative Control (K-), A. paniculata extract (EAP-200), and Nanoparticle of A. paniculata extract Group (nEAP-100, nEAP-200, and nEAP-400). Colony was measured using total plate count method. SOD & MDA level were measured using colorimetric method RESULT: L. monocytogenes bacterial count from group with extract A. paniculata was significantly lower than those in negative control (p<0,05). SOD level from group with extract A. paniculata was very significantly higher than those in negative control (p<0,01), while MDA level from group with extract A. paniculata was very significantly lower than those in negative control (p<0,01). Dose of 200 mg/kgBW in nanoparticle form was the most optimum dose. CONCLUSION: Extract of A. paniculata at various doses and form, increase bacterial clearance, increase SOD level, and reduce MDA level in rat infected with L. monocytogenes.
Atmaja et al. (Thu,) studied this question.